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验证数据展示

  • IF Staining of HepG2 using CL594-66910

    IF Staining of HepG2 using CL594-66910

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 INF2 antibody (CL594-66910, Clone: 4A11A7 ) at dilution of 1:200.

CoraLite®594-conjugated INF2 Monoclonal antibody

INF2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66910

Print datasheet

CloneNo. 4A11A7

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66910-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66910 targets INF2 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen INF2 fusion protein Ag17043 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name inverted formin, FH2 and WH2 domain containing
Synonyms C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484
Calculated Molecular Weight 1249 aa, 136 kDa
Observed Molecular Weight 150-170 kDa
GenBank Accession NumberBC008756
Gene Symbol INF2
Gene ID (NCBI) 64423
RRIDAB_2920055
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ27J81
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

实验方案

Product Specific Protocols
IF protocol for CL594 INF2 antibody CL594-66910Download protocol
Standard Protocols
Click here to view our Standard Protocols