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验证数据展示

  • WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

    IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING5 antibody included
  • IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

    IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ING5 Polyclonal antibody, PBS Only

ING5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10665-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ING5 Ready-To-Use IHC Kit. ING5 primary antibody included.

产品信息

10665-1-PBS targets ING5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ING5 fusion protein Ag1059 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibitor of growth family, member 5
Synonyms
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC005370
Gene Symbol ING5
Gene ID (NCBI) 84289
RRIDAB_2280522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYH8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)