INO80 Monoclonal antibody
INO80 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1F11C5
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | PC-12 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68064-1-Ig targets INO80 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | INO80 fusion protein Ag20265 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | INO80 homolog (S. cerevisiae) |
Synonyms | hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80 |
Calculated Molecular Weight | 1556 aa, 177 kDa |
Observed Molecular Weight | 177 kDa |
GenBank Accession Number | BC146785 |
Gene Symbol | INO80 |
Gene ID (NCBI) | 54617 |
RRID | AB_2918805 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9ULG1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for INO80 antibody 68064-1-Ig | Download protocol |
IHC protocol for INO80 antibody 68064-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |