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CoraLite® Plus 488-conjugated INO80 Monoclonal antibody

INO80 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F11C5

Cat no : CL488-68064

Print datasheet

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

验证数据展示

  • FC experiment of PC-12 using CL488-68064

    FC experiment of PC-12 using CL488-68064

    1X10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human INO80 (CL488-68064, Clone:1F11C5) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 68064-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68064 targets INO80 in FC (Intra) applications and shows reactivity with rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity rat
Immunogen INO80 fusion protein Ag20265 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name INO80 homolog (S. cerevisiae)
Synonyms hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80
Calculated Molecular Weight 1556 aa, 177 kDa
Observed Molecular Weight177 kDa
GenBank Accession NumberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULG1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 INO80 antibody CL488-68064Download protocol
Standard Protocols
Click here to view our Standard Protocols