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INPP5E Polyclonal antibody, PBS Only

INPP5E Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29353-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29353-1-AP (same clone as 29353-1-PBS)

    WB analysis using 29353-1-AP (same clone as 29353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

  • IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

  • IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

产品信息

29353-1-PBS targets INPP5E in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen INPP5E fusion protein Ag30305 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Synonyms
Calculated Molecular Weight 644 aa, 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC028032
Gene Symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.