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INPP5E Recombinant antibody

INPP5E Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E7

Cat no : 82689-3-RR

Print datasheet

Synonyms

Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, Inositol polyphosphate-5-phosphatase E, EC:3.1.3.86, EC:3.1.3.36, 72 kDa inositol polyphosphate 5-phosphatase

验证数据展示

  • IF Staining of hTERT-RPE1 using 82689-3-RR

    IF Staining of hTERT-RPE1 using 82689-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using INPP5E antibody (82689-3-RR, Clone: 1E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Affinity and Kinetic Characterization of 82689-3-RR

    Affinity and Kinetic Characterization of 82689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82689-3-RR against Human INPP5E were performed. The affinity constant is 0.166 nM.

经过测试的应用

Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82689-3-RR targets INPP5E in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen INPP5E fusion protein Ag30305 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Synonyms Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, Inositol polyphosphate-5-phosphatase E, EC:3.1.3.86, EC:3.1.3.36, 72 kDa inositol polyphosphate 5-phosphatase
Calculated Molecular Weight 644 aa, 70 kDa
Observed Molecular Weight 64-66 kDa
GenBank Accession NumberBC028032
Gene Symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

实验方案

Product Specific Protocols
IF protocol for INPP5E antibody 82689-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols