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INSR Recombinant antibody, PBS Only (Capture/Detector)

INSR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241687H7

Cat no : 84467-2-PBS

Print datasheet

Synonyms

IR, insulin receptor, HHF5, EC:2.7.10.1, CD220

验证数据展示

  • WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

    WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2, Human INSR Recombinant Matched Antibody Pair - PBS only. 84467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00007. 84467-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP01349-2

    Sample test of MP01349-2

    The mean INSR concentration was determined to be 72.33 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load and 87.72 ng/mL in HT-1080 cell extract based on a 2.50 mg/mL extract load.

  • Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1, INSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84467-2-PBS. Detection antibody: 84467-1-PBS. Standard: RP00007. Range: 1.56-100 ng/mL.

产品信息

84467-2-PBS targets INSR as part of a matched antibody pair:

MP01349-1: 84467-2-PBS capture and 84467-1-PBS detection (validated in Cytometric bead array)

MP01349-2: 84467-1-PBS capture and 84467-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name INSR
Synonyms IR, insulin receptor, HHF5, EC:2.7.10.1, CD220
Observed Molecular Weight95 kDa
GenBank Accession NumberBC117172
Gene Symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).