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验证数据展示

  • WB analysis using 84467-2-RR

    WB analysis using 84467-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84467-2-RR

    Affinity and Kinetic Characterization of 84467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

INSR Recombinant antibody

INSR Recombinant Antibody for WB, ELISA
Cat No. 84467-2-RR

产品说明书

CloneNo. 241687H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

IR, insulin receptor, HHF5, EC:2.7.10.1, CD220

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84467-2-RR targets INSR in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 INSR
别名 IR, insulin receptor, HHF5, EC:2.7.10.1, CD220
观测分子量95 kDa
GenBank蛋白编号BC117172
基因名称 INSR
Gene ID (NCBI) 3643
RRIDAB_3671990
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

实验方案

Product Specific Protocols
WB protocol for INSR antibody 84467-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols