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Anti-Human INSR/CD220 Rabbit Recombinant Antibody, PBS Only

INSR/CD220 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241687A10

Cat no : 98227-1-PBS

Print datasheet

Synonyms

INSR, insulin receptor, HHF5, EC:2.7.10.1, CD220

验证数据展示

  • FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

    FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone: 241687A10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were not fixed. This data was developed using the same antibody clone with 98227-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

    FC experiment of human PBMCs using 98227-1-RR (same clone as 98227-1-PBS)

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone:241687A10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98227-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98227-1-RR

    Affinity and Kinetic Characterization of 98227-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98227-1-RR against Human INSR/CD220 were performed. The affinity constant is 52.4 pM.

产品信息

98227-1-PBS targets INSR/CD220 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name INSR
Synonyms INSR, insulin receptor, HHF5, EC:2.7.10.1, CD220
GenBank Accession NumberBC117172
Gene Symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).