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INTS4 Recombinant antibody

INTS4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240579H4

Cat no : 83590-5-RR

Print datasheet

Synonyms

MSTP093, MST093, Int4, 240579H4

验证数据展示

  • IF Staining of U2OS using 83590-5-RR

    IF Staining of U2OS using 83590-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83590-5-RR

    FC experiment of A431 using 83590-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug INTS4 Recombinant antibody (83590-5-RR, Clone:240579H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83590-5-RR

    Affinity and Kinetic Characterization of 83590-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83590-5-RR against Human INTS4 were performed. The affinity constant is 0.146 nM.

经过测试的应用

Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83590-5-RR is INTS4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen INTS4 fusion protein Ag9116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrator complex subunit 4
Synonyms MSTP093, MST093, Int4, 240579H4
Calculated Molecular Weight963aa,108 kDa; 505aa,57 kDa
GenBank Accession NumberBC009995
Gene Symbol INTS4
Gene ID (NCBI) 92105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

实验方案

Product Specific Protocols
IF protocol for INTS4 antibody 83590-5-RRDownload protocol
FC protocol for INTS4 antibody 83590-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols