• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Sandwich ELISA standard curve of MP01446-1

    Sandwich ELISA standard curve of MP01446-1

    Sandwich ELISA standard curve of MP01446-1, Human INTS6 Recombinant Matched Antibody Pair - PBS only. 84610-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36146. 84610-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01446-1

    Sample test of MP01446-1

    The mean INTS6 concentration was determined to be 61.44 ng/mL in DU 145 cell extract based on a 1.00 mg/mL extract load.

  • IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

    IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

    IHC staining of mouse liver using 84610-3-RR (same clone as 84610-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84610-3-RR (same clone as 84610-3-PBS)

    IF Staining of HeLa using 84610-3-RR (same clone as 84610-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-3-RR, Clone: 242099E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 84610-3-RR (same clone as 84610-3-PBS)

    FC experiment of U-251 using 84610-3-RR (same clone as 84610-3-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug INTS6 Recombinant antibody (84610-3-RR, Clone:242099E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84610-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01446-1

    Cytometric bead array standard curve of MP01446-1

    Cytometric bead array standard curve of MP01446-1, INTS6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84610-3-PBS. Detection antibody: 84610-1-PBS. Standard: Ag36146. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01446-2

    Cytometric bead array standard curve of MP01446-2

    Cytometric bead array standard curve of MP01446-2, INTS6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84610-3-PBS. Detection antibody: 84610-2-PBS. Standard: Ag36146. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84610-3-RR

    Affinity and Kinetic Characterization of 84610-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-3-RR against Human INTS6 were performed. The affinity constant is 1.71 nM.

INTS6 Recombinant antibody, PBS Only (Capture)

INTS6 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84610-3-PBS

产品说明书

CloneNo. 242099E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

DICE1, DDX26A, DDX26, DBI1, DBI 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84610-3-PBS targets INTS6 as part of a matched antibody pair:

MP01446-1: 84610-3-PBS capture and 84610-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01446-2: 84610-3-PBS capture and 84610-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 INTS6 fusion protein Ag36146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 6
别名 DICE1, DDX26A, DDX26, DBI1, DBI 1
计算分子量 100 kDa
GenBank蛋白编号BC039829
基因名称 INTS6
Gene ID (NCBI) 26512
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UL03
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.