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IRAK1 Monoclonal antibody

IRAK1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66653-1-Ig

产品说明书

CloneNo. 1H10A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human and More (1)

应用

WB, IHC, ELISA and More (1)

IRAK, IRAK 1, IRAK1, pelle

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRAK1 Ready-To-Use IHC Kit. IRAK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66653-1-Ig targets IRAK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 Human
文献引用反应性human
免疫原 IRAK1 fusion protein Ag0728 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 1
别名 IRAK, IRAK 1, IRAK1, pelle
计算分子量 77 kDa
观测分子量 80 kDa
GenBank蛋白编号BC014963
基因名称 IRAK1
Gene ID (NCBI) 3654
RRIDAB_2882010
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51617
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

实验方案

Product Specific Protocols
WB protocol for IRAK1 antibody 66653-1-IgDownload protocol
IHC protocol for IRAK1 antibody 66653-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Mol Immunol

Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation.

Authors - Gang Xu
humanIF

Cell Death Dis

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells

Authors - Jing Li
humanIHC

Biol Reprod

miR-146b-5p downregulates IRAK1 and ADAM19 to suppress trophoblast proliferation, invasion and migration in miscarriage

Authors - Xiaoli Zhang
humanIF

J Cell Biol

BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation

Authors - Wei-Wei Li
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