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  • Featured Product
  • KD/KO Validated

IRAK1 Monoclonal antibody, PBS Only

IRAK1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10A7

Cat no : 66653-1-PBS

Print datasheet

Synonyms

IRAK, IRAK 1, IRAK1, pelle

验证数据展示

  • WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

    WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRAK1 antibody included
  • IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRAK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy IRAK1 Ready-To-Use IHC Kit. IRAK1 primary antibody included.

产品信息

66653-1-PBS targets IRAK1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen IRAK1 fusion protein Ag0728 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin-1 receptor-associated kinase 1
Synonyms IRAK, IRAK 1, IRAK1, pelle
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014963
Gene Symbol IRAK1
Gene ID (NCBI) 3654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51617
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)