验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, THP-1 cells, HT-29 cells, RAW 264.7 cells, zebrafish tissue, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80519-1-RR targets IRF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, zebrafish samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, zebrafish |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | interferon regulatory factor 3 |
| Synonyms | IRF-3, IRF 3, interferon regulatory factor 3, 2H6 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC009395 |
| Gene Symbol | IRF3 |
| Gene ID (NCBI) | 3661 |
| RRID | AB_2918899 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF3 antibody 80519-1-RR | Download protocol |
| IHC protocol for IRF3 antibody 80519-1-RR | Download protocol |
| IF protocol for IRF3 antibody 80519-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
