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IRF3 Recombinant antibody, PBS Only

IRF3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H6

Cat no : 80519-1-PBS

Print datasheet

Synonyms

IRF-3, IRF 3, interferon regulatory factor 3, 2H6

验证数据展示

  • WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

    WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 80519-1-RR (same clone as 80519-1-PBS)

    WB analysis of RAW 264.7 using 80519-1-RR (same clone as 80519-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

    IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF3 antibody included
  • IF Staining of HeLa using 80519-1-RR (same clone as 80519-1-PBS)

    IF Staining of HeLa using 80519-1-RR (same clone as 80519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IRF3 antibody (80519-1-RR, Clone: 2H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

产品信息

80519-1-PBS targets IRF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, zebrafish samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon regulatory factor 3
Synonyms IRF-3, IRF 3, interferon regulatory factor 3, 2H6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14653
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.