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验证数据展示

  • WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

    WB analysis using 80519-1-RR (same clone as 80519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 80519-1-RR (same clone as 80519-1-PBS)

    WB analysis of RAW 264.7 using 80519-1-RR (same clone as 80519-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

    IHC staining of human breast cancer using 80519-1-RR (same clone as 80519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF3 antibody included
  • IF Staining of HeLa using 80519-1-RR (same clone as 80519-1-PBS)

    IF Staining of HeLa using 80519-1-RR (same clone as 80519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IRF3 antibody (80519-1-RR, Clone: 2H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80519-1-PBS in a different storage buffer formulation.

IRF3 Recombinant antibody, PBS Only

IRF3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80519-1-PBS

产品说明书

CloneNo. 2H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, zebrafish

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

IRF-3, IRF 3, interferon regulatory factor 3, 2H6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

产品信息

80519-1-PBS targets IRF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, zebrafish samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon regulatory factor 3
别名 IRF-3, IRF 3, interferon regulatory factor 3, 2H6
计算分子量 47 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC009395
基因名称 IRF3
Gene ID (NCBI) 3661
RRIDAB_2918899
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14653
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.