IRF4 Monoclonal antibody
IRF4 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig and More (1)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1A12D5
验证数据展示
经过测试的应用
| Positive WB detected in | U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells |
| Positive IHC detected in | human tonsillitis Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66451-1-Ig targets IRF4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | IRF4 fusion protein Ag25203 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | IRF 4 |
| Synonyms | MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 48-52 kDa |
| GenBank Accession Number | BC015752 |
| Gene Symbol | IRF4 |
| Gene ID (NCBI) | 3662 |
| ENSEMBL Gene ID | ENSG00000137265 |
| RRID | AB_2881820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| IHC protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioeng Transl Med Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis. | ||
Biochim Biophys Acta Mol Cell Res RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4 |
