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验证数据展示

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve ofMP50012-1, IRF4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66451-2-PBS. Detection antibody: HRP-conjugated 66451-1-PBS. Standard: Ag25203. Range: 0.02-20 ng/mL.

IRF4 Monoclonal antibody, PBS Only (Detector)

IRF4 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 66451-1-PBS

产品说明书

CloneNo. 1A12D5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, Sandwich ELISA, Indirect ELISA

别名

MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

产品信息

66451-1-PBS targets IRF4 as part of a matched antibody pair:

MP50012-1: 66451-2-PBS capture and 66451-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 IRF4 fusion protein Ag25203 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IRF 4
别名 MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4
计算分子量 52 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC015752
基因名称 IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
RRIDAB_2881820
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15306
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.