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IRF4 Monoclonal antibody, PBS Only (Detector)

IRF4 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12D5

Cat no : 66451-1-PBS

Print datasheet

Synonyms

MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4

验证数据展示

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve ofMP50012-1, IRF4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66451-2-PBS. Detection antibody: HRP-conjugated 66451-1-PBS. Standard: Ag25203. Range: 0.02-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

产品信息

66451-1-PBS targets IRF4 as part of a matched antibody pair:

MP50012-1: 66451-2-PBS capture and 66451-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human, rat, pig
Immunogen IRF4 fusion protein Ag25203 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IRF 4
Synonyms MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15306
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.