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验证数据展示

  • IF Staining of THP-1 using CL594-67142

    IF Staining of THP-1 using CL594-67142

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 594 IRF8 antibody (CL594-67142, Clone: 3A10A4 ) at dilution of 1:200.

CoraLite® Plus 594-conjugated IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for IF/ICC
Cat No. CL594-67142

产品说明书

CloneNo. 3A10A4

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC

别名

H ICSBP, ICSBP, ICSBP1, interferon regulatory factor 8, IRF 8, IRF8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 67142-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67142 targets IRF8 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 IRF8 fusion protein Ag19909 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ICSBP1
别名 H ICSBP, ICSBP, ICSBP1, interferon regulatory factor 8, IRF 8, IRF8
计算分子量 426 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC126247
基因名称 IRF8
Gene ID (NCBI) 3394
RRIDAB_3084750
偶联类型 CoraLite® Plus 594 Fluorescent Dye
最大激发/发射波长594 nm / 615 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02556
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 594 IRF8 antibody CL594-67142Download protocol
Standard Protocols
Click here to view our Standard Protocols