- Featured Product
- KD/KO Validated
ISG15 Polyclonal antibody
ISG15 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, A549 cells, MCF-7 cells, PC-3 cells, IFN alpha treated A459 cells, HepG2 cells, IFN alpha treated HepG2 cells |
Positive IHC detected in | human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15981-1-AP targets ISG15 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF, IHC, WB, ELISA |
Cited Applications | CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, goat, monkey, pig |
Immunogen | ISG15 fusion protein Ag8782 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ISG15 ubiquitin-like modifier |
Synonyms | G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP |
Calculated Molecular Weight | 165 aa, 18 kDa |
Observed Molecular Weight | 15-17 kDa |
GenBank Accession Number | BC009507 |
Gene Symbol | ISG15 |
Gene ID (NCBI) | 9636 |
RRID | AB_2126302 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05161 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ISG15 antibody 15981-1-AP | Download protocol |
IHC protocol for ISG15 antibody 15981-1-AP | Download protocol |
IF protocol for ISG15 antibody 15981-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Commun Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease. | ||
Nat Commun ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins. | ||
J Exp Clin Cancer Res ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma
| ||
Cancer Res ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells. | ||