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ISLR Recombinant antibody

ISLR Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240727D4

Cat no : 83782-5-RR

Print datasheet

Synonyms

UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4

验证数据展示

  • WB analysis using 83782-5-RR

    WB analysis using 83782-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83782-5-RR

    Affinity and Kinetic Characterization of 83782-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83782-5-RR against Human ISLR were performed. The affinity constant is 0.369 nM.

经过测试的应用

Positive WB detected inmouse retina tissue, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83782-5-RR targets ISLR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ISLR fusion protein Ag19446 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name immunoglobulin superfamily containing leucine-rich repeat
Synonyms UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4
Calculated Molecular Weight 428 aa, 46 kDa
Observed Molecular Weight44-46 kDa
GenBank Accession NumberBC022478
Gene Symbol ISLR
Gene ID (NCBI) 3671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14498
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

实验方案

Product Specific Protocols
WB protocol for ISLR antibody 83782-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols