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验证数据展示

  • WB analysis using 30010-1-AP

    WB analysis using 30010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30010-1-AP (ITGBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ITGBL1 Polyclonal antibody

ITGBL1 Polyclonal Antibody for WB, ELISA
Cat No. 30010-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Integrin beta like protein 1, ITGBL1, OSCP, TIED

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SKOV-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30010-1-AP targets ITGBL1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ITGBL1 fusion protein Ag31989 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, beta-like 1 (with EGF-like repeat domains)
别名 Integrin beta like protein 1, ITGBL1, OSCP, TIED
计算分子量 494 aa, 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC036788
基因名称 ITGBL1
Gene ID (NCBI) 9358
RRIDAB_3086208
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95965
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-like protein 1 (ITGBL1), also named as TIED or OSCP, is a secreted protein that contains ten tandem EGF-like repeats strikingly similar to those found in the cysteine-rich "stalk-like" structure of integrin beta subunits (PMID: 10051402). ITGBL1 participates in the regulation of fibrogenesis via interactions with transforming growth factor beta 1 (PMID: 28262670). ITGBL1 is also involved in the development and metastasis of many tumors (PMID: 32211321; 29772438; 26060017; 31190876).

实验方案

Product Specific Protocols
WB protocol for ITGBL1 antibody 30010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols