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验证数据展示

  • WB analysis using 21525-1-AP

    WB analysis using 21525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 21525-1-AP

    IF Staining of Jurkat using 21525-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (21525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ITK Polyclonal antibody

ITK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21525-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Kinase EMT, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, IL2 inducible T cell kinase, EMT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse spleen tissue
Positive IF/ICC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21525-1-AP targets ITK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ITK fusion protein Ag16132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IL2-inducible T-cell kinase
别名 Kinase EMT, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, IL2 inducible T cell kinase, EMT
计算分子量 620 aa, 72 kDa
观测分子量 68-72 kDa
GenBank蛋白编号BC109077
基因名称 ITK
Gene ID (NCBI) 3702
RRIDAB_10733884
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08881
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITK antibody 21525-1-APDownload protocol
IF protocol for ITK antibody 21525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Role of Signal-Transducing Adaptor Protein-1 for T Cell Activation and Pathogenesis of Autoimmune Demyelination and Airway Inflammation

Authors - Kota Kagohashi
View Article
humanWB

Cytotherapy

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma

Authors - Wenjing Luo
View Article