验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 21525-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-21525 targets ITK in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ITK fusion protein Ag16132 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IL2-inducible T-cell kinase |
| 别名 | EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk |
| 计算分子量 | 620 aa, 72 kDa |
| 观测分子量 | 68-72 kDa |
| GenBank蛋白编号 | BC109077 |
| 基因名称 | ITK |
| Gene ID (NCBI) | 3702 |
| RRID | AB_2919639 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08881 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 ITK antibody CL555-21525 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |