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CoraLite®555-conjugated ITK Polyclonal antibody

ITK Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-21525

Print datasheet

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

验证数据展示

  • IF Staining of Jurkat using CL555-21525

    IF Staining of Jurkat using CL555-21525

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®555 ITK antibody (CL555-21525) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 21525-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL555-21525 is ITK Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ITK fusion protein Ag16132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IL2-inducible T-cell kinase
Synonyms EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_2919639
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08881
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 ITK antibody CL555-21525Download protocol
Standard Protocols
Click here to view our Standard Protocols