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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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CoraLite® Plus 647-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-30000

Print datasheet

Synonyms

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

验证数据展示

  • FC experiment of HeLa using CL647-30000

    FC experiment of HeLa using CL647-30000

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Rabbit IgG control (CL647-30000) (red), or 0.2 ug CoraLite® Plus 647-conjugated TRAP1 Rabbit PolyAb (CL647-10325) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-30000 targets Rabbit IgG control in FC (Intra) applications and shows reactivity with n/a samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity n/a
Immunogen N/A 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rabbit IgG control
Synonyms Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883649
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. This antibody can be used as Rabbit IgG control in experiments.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Rabbit IgG control antibody CL647-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols