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FITC Plus Rat IgG2b Isotype Control (LTF-2)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

LTF-2

Cat no : FITC-65211

Print datasheet

Synonyms

FITC Rat IgG2b Isotype Control, IgG2b, IgG2b Isotype Control, Rat IgG2b Isotype Control

验证数据展示

  • FC experiment of mouse splenocytes using FITC-65211

    FC experiment of mouse splenocytes using FITC-65211

    1X10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Rat IgG2b Isotype Control (FITC-65211, Clone:LTF-2) (red) or 0.25 ug FITC Plus Anti-Mouse CD4 (GK1.5) (green) or unstained (blue). Cells were not fixed.

经过测试的应用

Positive FC detected in

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

FITC-65211 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Cited ApplicationsFC
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name IgG2b Isotype Control
Synonyms FITC Rat IgG2b Isotype Control, IgG2b, IgG2b Isotype Control, Rat IgG2b Isotype Control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883825
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for FITC Plus IgG2b Isotype Control antibody FITC-65211Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
FC

PLoS One

Lipopolysaccharides derived from Porphyromonas gingivalis and Escherichia coli: Differential and interactive effects on novelty-induced hyperlocomotion, blood cytokine levels and TLR4-related processes

Authors - Yuri Aono
View Article