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验证数据展示

  • WB analysis using 68999-1-Ig

    WB analysis using 68999-1-Ig

    Untreated PC-3 cells, PC-3 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

  • WB analysis using 68999-1-Ig

    WB analysis using 68999-1-Ig

    Untreated MCF-7 cells, MCF-7 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control.

Phospho-IkB Alpha (Ser32/36) Monoclonal antibody

Phospho-IkB Alpha (Ser32/36) Monoclonal Antibody for WB, ELISA
Cat No. 68999-1-Ig

Print datasheet

CloneNo. 2G8E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

P IkB alpha, P IκB α, Pho-IkB Alpha, Pho-IkBα, Phospho IkB Alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, MCF-7 cells, Calyculin A treated MCF-7 cells, Calyculin A treated PC cells
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68999-1-Ig targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms P IkB alpha, P IκB α, Pho-IkB Alpha, Pho-IkBα, Phospho IkB Alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC004983
Gene Symbol IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_3670459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25963
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).

实验方案

Product Specific Protocols
WB protocol for Phospho-IkB Alpha (Ser32/36) antibody 68999-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols