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验证数据展示

  • WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

    WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

    Untreated PC-3 cells, PC-3 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control. This data was developed using the same antibody clone with 68999-1-PBS in a different storage buffer formulation.

  • WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

    WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

    Untreated MCF-7 cells, MCF-7 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control. This data was developed using the same antibody clone with 68999-1-PBS in a different storage buffer formulation.

Phospho-IkB Alpha (Ser32/36) Monoclonal antibody, PBS Only

Phospho-IkB Alpha (Ser32/36) Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68999-1-PBS

产品说明书

CloneNo. 2G8E5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Indirect ELISA

别名

IKBA, IkB alpha, IκB α, NFKBIA, I-kappa-B-alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

68999-1-PBS targets Phospho-IkB Alpha (Ser32/36) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 IKBA, IkB alpha, IκB α, NFKBIA, I-kappa-B-alpha
计算分子量 36 kDa
观测分子量 36-39 kDa
GenBank蛋白编号BC004983
基因名称 IkB Alpha
Gene ID (NCBI) 4792
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25963
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).