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验证数据展示

  • WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

    WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80019-1-RR (IkB Alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human colon cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80019-1-RR (IkB Alpha antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • Affinity and Kinetic Characterization of 80019-1-RR

    Affinity and Kinetic Characterization of 80019-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80019-1-RR against Human IkB Alpha were performed. The affinity constant is below 1 pM.

IkB Alpha Recombinant antibody, PBS Only

IkB Alpha Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80019-1-PBS

产品说明书

CloneNo. 5C24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

IKBA, IκB α, NFKBIA, 5C24, I kappa B alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

80019-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IkB Alpha fusion protein Ag0345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 IKBA, IκB α, NFKBIA, 5C24, I kappa B alpha
计算分子量 36 kDa
观测分子量36 kDa and 35 kDa
GenBank蛋白编号BC002601
基因名称 IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2882942
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25963
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.