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IkB Alpha Recombinant antibody, PBS Only

IkB Alpha Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C24

Cat no : 80019-1-PBS

Print datasheet

Synonyms

IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha

验证数据展示

  • WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

    WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • Affinity and Kinetic Characterization of 80019-1-RR

    Affinity and Kinetic Characterization of 80019-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80019-1-RR against Human IkB Alpha were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

80019-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IkB Alpha fusion protein Ag0345 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight36 kDa and 35 kDa
GenBank Accession NumberBC002601
Gene Symbol IkB Alpha
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25963
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.