IkB Alpha Recombinant antibody
IkB Alpha Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5C24
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, Raji cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80019-1-RR targets IkB Alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | IkB Alpha fusion protein Ag0345 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Synonyms | IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa and 35 kDa |
| GenBank Accession Number | BC002601 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2882942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IkB Alpha antibody 80019-1-RR | Download protocol |
| IHC protocol for IkB Alpha antibody 80019-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
