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验证数据展示

  • IF Staining of HeLa using CL647-10268

    IF Staining of HeLa using CL647-10268

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 IkB Alpha antibody (CL647-10268) at dilution of 1:200.

CoraLite® Plus 647-conjugated IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for IF/ICC
Cat No. CL647-10268

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig, rat

应用

IF/ICC

别名

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10268-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10268 targets IkB Alpha in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, pig, rat
免疫原 IkB Alpha fusion protein Ag0345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA
计算分子量 36 kDa
GenBank蛋白编号BC002601
基因名称 IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2920209
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25963
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 IkB Alpha antibody CL647-10268Download protocol
Standard Protocols
Click here to view our Standard Protocols