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验证数据展示

  • WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67897-1-Ig (same clone as 67897-1-PBS)

    IHC staining of mouse testis using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67897-1-Ig (Importin Alpha 5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KPNA1 antibody included
  • WB analysis of HeLa using 67897-1-Ig (same clone as 67897-1-PBS)

    WB analysis of HeLa using 67897-1-Ig (same clone as 67897-1-PBS)

    WB result of Importin Alpha 5 antibody (67897-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HeLa cells. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Importin Alpha 5 Monoclonal antibody, PBS Only

Importin Alpha 5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67897-1-PBS

产品说明书

CloneNo. 2A7G7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.

产品信息

67897-1-PBS targets Importin Alpha 5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Importin Alpha 5 fusion protein Ag12751 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 karyopherin alpha 1 (importin alpha 5)
别名 Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta
计算分子量 60 kDa
观测分子量60-62 kDa
GenBank蛋白编号BC002374
基因名称 Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2918653
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52294
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.