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  • Featured Product
  • KD/KO Validated

Importin Alpha 5 Monoclonal antibody, PBS Only

Importin Alpha 5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7G7

Cat no : 67897-1-PBS

Print datasheet

Synonyms

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

验证数据展示

  • WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67897-1-Ig (same clone as 67897-1-PBS)

    IHC staining of mouse testis using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67897-1-Ig (Importin Alpha 5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KPNA1 antibody included
  • WB analysis of HeLa using 67897-1-Ig (same clone as 67897-1-PBS)

    WB analysis of HeLa using 67897-1-Ig (same clone as 67897-1-PBS)

    WB result of Importin Alpha 5 antibody (67897-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HeLa cells. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.

产品信息

67897-1-PBS targets Importin Alpha 5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Importin Alpha 5 fusion protein Ag12751 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 1 (importin alpha 5)
Synonyms Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60-62 kDa
GenBank Accession NumberBC002374
Gene Symbol Importin Alpha 5
Gene ID (NCBI) 3836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52294
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.