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验证数据展示

  • WB analysis using 67897-1-Ig

    WB analysis using 67897-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of MCF-7 using 67897-1-Ig

    IF Staining of MCF-7 using 67897-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse testis using 67897-1-Ig

    IHC staining of mouse testis using 67897-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67897-1-Ig (Importin Alpha 5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA1 antibody included
  • WB analysis of HeLa using 67897-1-Ig

    WB analysis of HeLa using 67897-1-Ig

    WB result of Importin Alpha 5 antibody (67897-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HeLa cells.

  • IF Staining of MCF-7 using 67897-1-Ig

    IF Staining of MCF-7 using 67897-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

Importin Alpha 5 Monoclonal antibody

Importin Alpha 5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67897-1-Ig

产品说明书

CloneNo. 2A7G7
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67897-1-Ig targets Importin Alpha 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 Importin Alpha 5 fusion protein Ag12751 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 karyopherin alpha 1 (importin alpha 5)
别名 Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta
计算分子量 60 kDa
观测分子量60-62 kDa
GenBank蛋白编号BC002374
基因名称 Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2918653
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

实验方案

Product Specific Protocols
WB protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
IHC protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
IF protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

bioRxiv

Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA

Authors - Patrick James Sutton
View Article