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验证数据展示

  • FC experiment of MCF-7 using CL488-67897

    FC experiment of MCF-7 using CL488-67897

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Importin Alpha 5 (CL488-67897, Clone:2A7G7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Importin Alpha 5 Monoclonal antibody

Importin Alpha 5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67897

产品说明书

CloneNo. 2A7G7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

IMA5, KPNA1, 2A7G7, Impα5, Importin subunit alpha-5, N-terminally processed

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67897-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67897 targets Importin Alpha 5 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 Importin Alpha 5 fusion protein Ag12751 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 karyopherin alpha 1 (importin alpha 5)
别名 IMA5, KPNA1, 2A7G7, Impα5, Importin subunit alpha-5, N-terminally processed
计算分子量 60 kDa
观测分子量60-62 kDa
GenBank蛋白编号BC002374
基因名称 Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2923820
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Importin Alpha 5 antibody CL488-67897Download protocol
Standard Protocols
Click here to view our Standard Protocols