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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Importin Alpha 5 Monoclonal antibody

Importin Alpha 5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A7G7

Cat no : CL488-67897

Print datasheet

Synonyms

IMA5, KPNA1, 2A7G7, Impα5, Importin subunit alpha-5, N-terminally processed

验证数据展示

  • FC experiment of MCF-7 using CL488-67897

    FC experiment of MCF-7 using CL488-67897

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Importin Alpha 5 (CL488-67897, Clone:2A7G7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67897-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67897 targets Importin Alpha 5 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen Importin Alpha 5 fusion protein Ag12751 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 1 (importin alpha 5)
Synonyms IMA5, KPNA1, 2A7G7, Impα5, Importin subunit alpha-5, N-terminally processed
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60-62 kDa
GenBank Accession NumberBC002374
Gene Symbol Importin Alpha 5
Gene ID (NCBI) 3836
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52294
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Importin Alpha 5 antibody CL488-67897Download protocol
Standard Protocols
Click here to view our Standard Protocols