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验证数据展示

  • IF Staining of HepG2 using CL594-67897

    IF Staining of HepG2 using CL594-67897

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Importin Alpha 5 antibody (CL594-67897, Clone: 2A7G7 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite®594-conjugated Importin Alpha 5 Monoclonal antibody

Importin Alpha 5 Monoclonal Antibody for IF/ICC
Cat No. CL594-67897

产品说明书

CloneNo. 2A7G7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA1 Ready-To-Use IHC Kit. KPNA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67897-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67897 targets Importin Alpha 5 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 Importin Alpha 5 fusion protein Ag12751 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 karyopherin alpha 1 (importin alpha 5)
别名 Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta
计算分子量 60 kDa
观测分子量60-62 kDa
GenBank蛋白编号BC002374
基因名称 Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2923932
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

实验方案

Product Specific Protocols
IF protocol for CL594 Importin Alpha 5 antibody CL594-67897Download protocol
Standard Protocols
Click here to view our Standard Protocols