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Importin Beta Monoclonal antibody, PBS Only

Importin Beta Monoclonal Antibody for WB,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

1A12B1

Cat No : 67597-1-PBS

Print datasheet

Synonyms

IMB1, Impnb, Importin 90, Importin Beta, Importin subunit beta 1, IPO1, IPOB, karyopherin (importin) beta 1, Karyopherin subunit beta 1, KPNB1, NTF97, Nuclear factor p97, PTAC97

验证数据展示

  • IHC staining of human colon cancer using 67597-1-Ig (same clone as 67597-1-PBS)

    IHC staining of human colon cancer using 67597-1-Ig (same clone as 67597-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KPNB1 antibody included
  • WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

    WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67597-1-Ig (same clone as 67597-1-PBS)

    FC experiment of Jurkat using 67597-1-Ig (same clone as 67597-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.

产品信息

67597-1-PBS targets Importin Beta in WB, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,FC Application Description
Tested Reactivity Human
Immunogen Importin Beta fusion protein Ag23094 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name karyopherin (importin) beta 1
Synonyms IMB1, Impnb, Importin 90, Importin Beta, Importin subunit beta 1, IPO1, IPOB, karyopherin (importin) beta 1, Karyopherin subunit beta 1, KPNB1, NTF97, Nuclear factor p97, PTAC97
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 95-97 kDa
GenBank Accession NumberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14974
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)