验证数据展示
经过测试的应用
Positive WB detected in | IMR-32 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, A549 cells, Caco-2 cells, MDA-MB-231 cells, MOLT-4 |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67597-1-Ig targets Importin Beta in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | Importin Beta fusion protein Ag23094 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | karyopherin (importin) beta 1 |
别名 | KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb |
计算分子量 | 97 kDa |
观测分子量 | 95-97 kDa |
GenBank蛋白编号 | BC003572 |
基因名称 | KPNB1 |
Gene ID (NCBI) | 3837 |
RRID | AB_2882803 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q14974 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Importin Beta antibody 67597-1-Ig | Download protocol |
IHC protocol for Importin Beta antibody 67597-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |