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验证数据展示

  • IHC staining of human colon cancer using 67597-1-Ig

    IHC staining of human colon cancer using 67597-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNB1 antibody included
  • WB analysis using 67597-1-Ig

    WB analysis using 67597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67597-1-Ig

    WB analysis using 67597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 67597-1-Ig

    FC experiment of Jurkat using 67597-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Importin Beta Monoclonal antibody

Importin Beta Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67597-1-Ig

Print datasheet

CloneNo. 1A12B1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inIMR-32 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, A549 cells, Caco-2 cells, MDA-MB-231 cells, MOLT-4
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67597-1-Ig targets Importin Beta in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Importin Beta fusion protein Ag23094 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name karyopherin (importin) beta 1
Synonyms KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 95-97 kDa
GenBank Accession NumberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
RRIDAB_2882803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
WB protocol for Importin Beta antibody 67597-1-IgDownload protocol
IHC protocol for Importin Beta antibody 67597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols