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验证数据展示

  • FC experiment of Jurkat using CL488-67597

    FC experiment of Jurkat using CL488-67597

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Importin Beta (CL488-67597, Clone:1A12B1) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Importin Beta Monoclonal antibody

Importin Beta Monoclonal Antibody for FC (Intra)
Cat No. CL488-67597

Print datasheet

CloneNo. 1A12B1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67597-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67597 targets Importin Beta in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Importin Beta fusion protein Ag23094 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name karyopherin (importin) beta 1
Synonyms KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 95-97 kDa
GenBank Accession NumberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
RRIDAB_3084363
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14974
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Importin Beta antibody CL488-67597Download protocol
Standard Protocols
Click here to view our Standard Protocols