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验证数据展示

  • WB analysis of rat pancreas using 83867-1-RR

    WB analysis of rat pancreas using 83867-1-RR

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 83867-1-RR (insulin1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse pancreas using 83867-1-RR

    IF Staining of mouse pancreas using 83867-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 83867-1-RR

    IF Staining of mouse pancreas using 83867-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using insulin1 antibody (83867-1-RR, Clone: 240798G4, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).SST-Specific antibody (84351-1-RR, Clone: 240816D9,red ) at dilution of 1:400 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 83867-1-RR

    IF Staining of mouse pancreas using 83867-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 83867-1-RR

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 83867-1-RR

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 83867-1-RR

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83867-1-RR

    Affinity and Kinetic Characterization of 83867-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83867-1-RR against Mouse Ins1 were performed. The affinity constant is 0.21 nM.

Ins1 Recombinant antibody

Ins1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83867-1-RR

产品说明书

CloneNo. 240798G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83867-1-RR targets Ins1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Ins1 fusion protein Ag29988 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin I
别名 Insulin, insulin1, insulin I, Ins2 rs1, Ins-1
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号NM_008386
基因名称 Ins1
Gene ID (NCBI) 16333
RRIDAB_3671453
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
WB protocol for Ins1 antibody 83867-1-RRDownload protocol
IHC protocol for Ins1 antibody 83867-1-RRDownload protocol
IF protocol for Ins1 antibody 83867-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols