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验证数据展示

  • IF Staining of mouse pancreas using CL488-83867

    IF Staining of mouse pancreas using CL488-83867

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 Ins1 antibody (CL488-83867, Clone: 240798G4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Ins1 Recombinant antibody

Ins1 Recombinant Antibody for IF-P
Cat No. CL488-83867

Print datasheet

CloneNo. 240798G4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF-P

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 83867-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83867 targets Ins1 in IF-P applications and shows reactivity with mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity mouse, rat
Immunogen Ins1 fusion protein Ag29988 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin I
Synonyms
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01325
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ins1 antibody CL488-83867Download protocol
Standard Protocols
Click here to view our Standard Protocols