验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse pancreas tissue |
For other applications, we recommend the unconjugated version of this antibody, 83867-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83867 targets Ins1 in IF-P applications and shows reactivity with mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 | Ins1 fusion protein Ag29988 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | insulin I |
| 别名 | |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | NM_008386 |
| 基因名称 | Ins1 |
| Gene ID (NCBI) | 16333 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01325 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Ins1 antibody CL488-83867 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |