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Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IF-P, IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Rat, mouse

Applications

IF-P, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3A11C1

Cat No : 67284-1-Ig

Print datasheet

Synonyms

Ins1, Ins-1

验证数据展示

  • IHC staining of rat pancreas using 67284-1-Ig

    IHC staining of rat pancreas using 67284-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67284-1-Ig (Insulin1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 67284-1-Ig

    IHC staining of rat pancreas using 67284-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67284-1-Ig (Insulin1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat pancreas using 67284-1-Ig

    IF Staining of rat pancreas using 67284-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Ins1 antibody (67284-1-Ig, Clone: 3A11C1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inrat pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat pancreas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:5000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67284-1-Ig targets Ins1 in IF, IHC, ELISA applications and shows reactivity with Rat, mouse samples.

Tested Applications IF-P, IHC,ELISA Application Description
Tested Reactivity Rat, mouse
Immunogen Ins1 fusion protein Ag28809 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name insulin 1
Synonyms Ins1, Ins-1
Calculated Molecular Weight 12 kDa
GenBank Accession NumberNM_019129.3
Gene Symbol Ins1
Gene ID (NCBI) 24505
RRIDAB_2882551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Ins1 antibody 67284-1-IgDownload protocol
IF protocol for Ins1 antibody 67284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols