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验证数据展示

  • WB analysis of HepG2 using 68211-1-Ig (same clone as 68211-1-PBS)

    WB analysis of HepG2 using 68211-1-Ig (same clone as 68211-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

    IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

    IF Staining of human placenta using 68211-1-Ig (same clone as 68211-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68211-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50423-3

    Cytometric bead array standard curve of MP50423-3

    Cytometric bead array standard curve of MP50423-3, Integrin alpha-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68211-4-PBS. Detection antibody: 68211-1-PBS. Standard:Ag30631. Range: 0.781-100 ng/mL.

Integrin alpha 1 Monoclonal antibody, PBS Only (Detector)

Integrin alpha 1 Monoclonal Antibody for WB, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 68211-1-PBS

产品说明书

CloneNo. 1C4F2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF-P, Cytometric bead array, Indirect ELISA

别名

ITGA1, Laminin and collagen receptor, integrin, alpha 1, CD49a, CD49 antigen-like family member A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Integrin alpha-1 Ready-To-Use IHC Kit. Integrin alpha-1 primary antibody included.

产品信息

68211-1-PBS targets Integrin alpha 1 as part of a matched antibody pair:

MP50423-3: 68211-4-PBS capture and 68211-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Integrin alpha 1 fusion protein Ag30631 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 1
别名 ITGA1, Laminin and collagen receptor, integrin, alpha 1, CD49a, CD49 antigen-like family member A
计算分子量 1179 aa, 131 kDa
观测分子量180 kDa
GenBank蛋白编号BC137121
基因名称 Integrin alpha 1
Gene ID (NCBI) 3672
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56199
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).