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Integrin alpha 2 Polyclonal antibody

Integrin alpha 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30703-1-AP

Print datasheet

Synonyms

ITGA2, VLA 2, GPIa, Collagen receptor, CD49B

验证数据展示

  • WB analysis using 30703-1-AP

    WB analysis using 30703-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30703-1-AP (Integrin alpha 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30703-1-AP

    IF Staining of A431 using 30703-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ITGA2 antibody (30703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHT-1080 cells, A431 cells, HeLa cells, MDA-MB-231 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30703-1-AP targets Integrin alpha 2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Integrin alpha 2 fusion protein Ag32771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Synonyms ITGA2, VLA 2, GPIa, Collagen receptor, CD49B
Calculated Molecular Weight126 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_002203
Gene Symbol Integrin alpha 2
Gene ID (NCBI) 3673
RRIDAB_3669752
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17301
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-2 (ITGA2, CD49b) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor (VLA-2) for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin.

实验方案

Product Specific Protocols
WB protocol for Integrin alpha 2 antibody 30703-1-APDownload protocol
IF protocol for Integrin alpha 2 antibody 30703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols