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验证数据展示

  • WB analysis using 84468-4-RR

    WB analysis using 84468-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84468-4-RR (Integrin alpha-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84468-4-RR

    IF Staining of HeLa using 84468-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Integrin alpha-5 antibody (84468-4-RR, Clone: 241685A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84468-4-RR

    Affinity and Kinetic Characterization of 84468-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84468-4-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.24 nM.

Integrin alpha-5 Recombinant antibody

Integrin alpha-5 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84468-4-RR

Print datasheet

CloneNo. 241685A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

ITGA5, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA, CD49e

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, human placenta tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84468-4-RR targets Integrin alpha-5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Synonyms ITGA5, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA, CD49e
Calculated Molecular Weight 115 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC008786
Gene Symbol Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_3671991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08648
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 84468-4-RRDownload protocol
IF protocol for Integrin alpha-5 antibody 84468-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols