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验证数据展示

  • IF Staining of HeLa using CL488-84468-4

    IF Staining of HeLa using CL488-84468-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Integrin alpha-5 antibody (CL488-84468-4, Clone: 241685A3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Integrin alpha-5 Recombinant antibody

Integrin alpha-5 Recombinant Antibody for IF/ICC
Cat No. CL488-84468-4

Print datasheet

CloneNo. 241685A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84468-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84468-4 targets Integrin alpha-5 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Synonyms
Calculated Molecular Weight 115 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC008786
Gene Symbol Integrin alpha 5
Gene ID (NCBI) 3678
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08648
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Integrin alpha-5 antibody CL488-84468-4Download protocol
Standard Protocols
Click here to view our Standard Protocols