验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, HeLa cells, A431 cells, U-87 MG cells, HCT 116 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | Customer Sample customer |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82729-3-RR targets Integrin Beta 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Integrin Beta 1 fusion protein Ag3078 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| Synonyms | ITGB1, Integrin beta-1, GPIIA, Glycoprotein IIa, FNRB |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 110-140 kDa |
| GenBank Accession Number | BC020057 |
| Gene Symbol | Integrin beta 1 |
| Gene ID (NCBI) | 3688 |
| ENSEMBL Gene ID | ENSG00000150093 |
| RRID | AB_3670539 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05556 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 1 antibody 82729-3-RR | Download protocol |
| IHC protocol for Integrin Beta 1 antibody 82729-3-RR | Download protocol |
| IF protocol for Integrin Beta 1 antibody 82729-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |