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Integrin beta-1 Recombinant antibody

Integrin beta-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1K20

Cat No : 82729-3-RR

Print datasheet

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

验证数据展示

  • WB analysis using 82729-3-RR

    WB analysis using 82729-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, A431 cells, U-87 MG cells, HCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82729-3-RR targets Integrin beta-1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Integrin beta-1 fusion protein Ag3078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Synonyms CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB
Calculated Molecular Weight 88 kDa
Observed Molecular Weight110-140 kDa
GenBank Accession NumberBC020057
Gene Symbol ITGB1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

实验方案

Product Specific Protocols
WB protocol for Integrin beta-1 antibody 82729-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols