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Integrin beta-6 Polyclonal antibody

Integrin beta-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28378-1-AP

Print datasheet

Synonyms

Integrin beta 6, Integrin beta-6, integrin, beta 6, ITGB6

验证数据展示

  • WB analysis using 28378-1-AP

    WB analysis using 28378-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 28378-1-AP

    IHC staining of mouse lung using 28378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGB6 antibody included
  • IHC staining of mouse lung using 28378-1-AP

    IHC staining of mouse lung using 28378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ITGB6 antibody included

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ITGB6 Ready-To-Use IHC Kit. ITGB6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28378-1-AP targets Integrin beta-6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen Integrin beta-6 fusion protein Ag29002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, beta 6
Synonyms Integrin beta 6, Integrin beta-6, integrin, beta 6, ITGB6
Calculated Molecular Weight 788 aa, 86 kDa
Observed Molecular Weight97 kDa
GenBank Accession NumberBC121178
Gene Symbol Integrin beta 6
Gene ID (NCBI) 3694
RRIDAB_2918157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

实验方案

Product Specific Protocols
WB protocol for Integrin beta-6 antibody 28378-1-APDownload protocol
IHC protocol for Integrin beta-6 antibody 28378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma

Authors - Junjian Yu
View Article
humanWB,IHC

Transl Lung Cancer Res

Exosomal ITGB6 from dormant lung adenocarcinoma cells activates cancer-associated fibroblasts by KLF10 positive feedback loop and the TGF-β pathway

Authors - Xiang Feng
View Article