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CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MPC-11

Cat no : CL647-65128

Print datasheet

Synonyms

CoraLite®647-conjugated Anti-Mouse Isotype Control, gamma2b, IgG2b, IgG2b Isotype Control, Igh-3, IGHG2B

验证数据展示

  • FC experiment of HepG2 using CL647-65128

    FC experiment of HepG2 using CL647-65128

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG2b Isotype Control (CL647-65128, Clone:MPC-11) (red), or 0.2 ug CoraLite® Plus 647-conjugated HDAC2 Mouse McAb (CL647-67165, Clone: 1A3E4) (green) or unstained (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive FC (Intra) detected in
Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 65128-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy chain 3 (serum IgG2b)
Synonyms CoraLite®647-conjugated Anti-Mouse Isotype Control, gamma2b, IgG2b, IgG2b Isotype Control, Igh-3, IGHG2B
Gene Symbol IgG2b Isotype Control
Gene ID (NCBI) 16016
RRIDAB_2883697
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01867
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.