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验证数据展示

  • FC experiment of HEK-293 using CL488-27511

    FC experiment of HEK-293 using CL488-27511

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JAB1 (CL488-27511) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated JAB1 Polyclonal antibody

JAB1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-27511

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

COPS5, SGN5, MOV 34, Jun activation domain-binding protein 1, EC:3.4.-.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COPS5/JAB1 Ready-To-Use IHC Kit. COPS5/JAB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27511-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27511 targets JAB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 JAB1 fusion protein Ag25950 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
别名 COPS5, SGN5, MOV 34, Jun activation domain-binding protein 1, EC:3.4.-.-
计算分子量 37 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC001187
基因名称 JAB1
Gene ID (NCBI) 10987
RRIDAB_3084109
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92905
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 JAB1 antibody CL488-27511Download protocol
Standard Protocols
Click here to view our Standard Protocols