JAK3 Recombinant antibody
JAK3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3N1
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 3 publications below |
产品信息
80331-1-RR targets JAK3 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | Janus kinase 3 (a protein tyrosine kinase, leukocyte) |
| Synonyms | JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Tyrosine protein kinase JAK3 |
| Calculated Molecular Weight | 1124 aa, 125 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | BC028068 |
| Gene Symbol | JAK3 |
| Gene ID (NCBI) | 3718 |
| RRID | AB_2918888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P52333 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JAK3 antibody 80331-1-RR | Download protocol |
| IHC protocol for JAK3 antibody 80331-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion | ||
Gene The JAK-STAT signaling-related signature serves as a prognostic and predictive biomarker for renal cell carcinoma immunotherapy | ||
Front Genet Identification of apoptosis-immune-related gene signature and construction of diagnostic model for sepsis based on single-cell sequencing and bulk transcriptome analysis | ||
Clin Exp Rheumatol Effectiveness of tofacitinib monotherapy for patients with IgG4-RD or idiopathic retroperitoneal fibrosis |
