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JMY Polyclonal antibody

JMY Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25098-1-AP

Print datasheet

Synonyms

WHDC1L3, Junction-mediating and -regulatory protein

验证数据展示

  • WB analysis of Jurkat using 25098-1-AP

    WB analysis of Jurkat using 25098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 25098-1-AP

    IP experiment of Jurkat using 25098-1-AP

    IP result of anti-JMY (IP:25098-1-AP, 4ug; Detection:25098-1-AP 1:600) with Jurkat cells lysate 2400ug.

  • IHC staining of human brain using 25098-1-AP

    IHC staining of human brain using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with JMY antibody included
  • IHC staining of human colon cancer using 25098-1-AP

    IHC staining of human colon cancer using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JMY antibody included
  • IHC staining of human colon cancer using 25098-1-AP

    IHC staining of human colon cancer using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JMY antibody included
  • IF Staining of HeLa using 25098-1-AP

    IF Staining of HeLa using 25098-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JMY antibody (25098-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy JMY Ready-To-Use IHC Kit. JMY primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25098-1-AP targets JMY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen JMY fusion protein Ag18735 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name junction mediating and regulatory protein, p53 cofactor
Synonyms WHDC1L3, Junction-mediating and -regulatory protein
Calculated Molecular Weight 988 aa, 111 kDa
Observed Molecular Weight 111 kDa
GenBank Accession NumberBC130624
Gene Symbol JMY
Gene ID (NCBI) 133746
RRIDAB_2879895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9B5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junction mediating and regulatory protein(JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.

实验方案

Product Specific Protocols
WB protocol for JMY antibody 25098-1-APDownload protocol
IHC protocol for JMY antibody 25098-1-APDownload protocol
IF protocol for JMY antibody 25098-1-APDownload protocol
IP protocol for JMY antibody 25098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS Genet

WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility.

Authors - Shail Kabrawala
View Article